Regulatory

Part:BBa_K3032112

Designed by: Gytis Druteika   Group: iGEM19_Vilnius-Lithuania   (2019-10-16)


EL222 regulated promoter based on Anderson (0.16)

This is an Anderson (0.16) promoter with EL222 binding site inside of it. Promoter is designed in such a way that EL222 repressor (BBa_K2332004) could precisely bind to it. EL222 DNA binding sequence was inserted into weak promoter’s region of -35 to -10.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 31
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None